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Addgene

pGA-blue
(Plasmid #196336)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196336 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGA-blue
  • Backbone manufacturer
    IndBioTech Lab
  • Backbone size (bp) 2709
  • Modifications to backbone
    The backbone was synthetically assembled from parts of other plasmids. It bears an AmilCP chromoprotien placeholder that is excided upon cloning
  • Vector type
    Synthetic Biology ; Golden Gate Assembly backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid was synthetically assembled from parts of other vectors: amilCP chromoprotein plasmid (a gift from Anthony Forster (Addgene plasmid # 117847 ; http://n2t.net/addgene:117847 ; RRID:Addgene_117847)); pStBlue-1 (Novagene)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGA-blue was a gift from Paola Branduardi (Addgene plasmid # 196336 ; http://n2t.net/addgene:196336 ; RRID:Addgene_196336)
  • For your References section:

    Easy Modular Integrative fuSion-ready Expression (Easy-MISE) Toolkit for Fast Engineering of Heterologous Productions in Saccharomyces cerevisiae. Maestroni L, Butti P, Milanesi R, Pagliari S, Campone L, Serra I, Branduardi P. ACS Synth Biol. 2023 Apr 14. doi: 10.1021/acssynbio.3c00015. 10.1021/acssynbio.3c00015 PubMed 37058502