pGA-blue
(Plasmid
#196336)
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Purpose(Empty Backbone) Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGA-blue
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Backbone manufacturerIndBioTech Lab
- Backbone size (bp) 2709
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Modifications to backboneThe backbone was synthetically assembled from parts of other plasmids. It bears an AmilCP chromoprotien placeholder that is excided upon cloning
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Vector typeSynthetic Biology ; Golden Gate Assembly backbone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid was synthetically assembled from parts of other vectors: amilCP chromoprotein plasmid (a gift from Anthony Forster (Addgene plasmid # 117847 ; http://n2t.net/addgene:117847 ; RRID:Addgene_117847)); pStBlue-1 (Novagene)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGA-blue was a gift from Paola Branduardi (Addgene plasmid # 196336 ; http://n2t.net/addgene:196336 ; RRID:Addgene_196336) -
For your References section:
Easy Modular Integrative fuSion-ready Expression (Easy-MISE) Toolkit for Fast Engineering of Heterologous Productions in Saccharomyces cerevisiae. Maestroni L, Butti P, Milanesi R, Pagliari S, Campone L, Serra I, Branduardi P. ACS Synth Biol. 2023 Apr 14. doi: 10.1021/acssynbio.3c00015. 10.1021/acssynbio.3c00015 PubMed 37058502