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Addgene

Tol2-TLCV2
(Plasmid #196331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196331 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pdesttol2
  • Backbone manufacturer
    Wang Wei
  • Backbone size w/o insert (bp) 4560
  • Total vector size (bp) 11509
  • Vector type
    Mammalian Expression, Bacterial Expression, Yeast Expression, CRISPR ; fish expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9-2A-eGFP
  • Insert Size (bp)
    6793
  • Promoter Tight TRE promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer Acatatccagtcactatggggtaccgccgcgaatcgatatgtcgagtttact
  • 3′ sequencing primer Gctgatcagcgggccgctttacccggggagcatgtcaaggtca
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    the TLCV2 was received from Addgene (#87360), the pDestTol2 backbone was received from Dr Wang Wei

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-TLCV2 was a gift from Itamar Harel (Addgene plasmid # 196331)

  • For your References section:

    A scalable and tunable platform for functional interrogation of peptide hormones in fish. Moses E, Franek R, Harel I. Elife. 2023 Oct 24;12:e85960. doi: 10.7554/eLife.85960. 10.7554/eLife.85960 PubMed 37872843
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