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Addgene

pTL-Bag2T-gRNA2
(Plasmid #196253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196253 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
  • gRNA/shRNA sequence
    no target sequence
  • Promoter J23119 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer 5-TGGCCCGTGTCTCAAAATCTCTG-3
  • 3′ sequencing primer 5'-ATCTTTTCTACGGGGTCTGACG-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTL-Bag2T-gRNA2 was a gift from Bing Yang (Addgene plasmid # 196253 ; http://n2t.net/addgene:196253 ; RRID:Addgene_196253)

  • For your References section:

    Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria. Li C, Wang L, Cseke LJ, Vasconcelos F, Huguet-Tapia JC, Gassmann W, Pauwels L, White FF, Dong H, Yang B. Commun Biol. 2023 Jan 17;6(1):56. doi: 10.1038/s42003-023-04451-8. 10.1038/s42003-023-04451-8 PubMed 36646768
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