pEN-dCas9-CDA1-UGI
(Plasmid
#196248)
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PurposePlasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameattL4-RecApro-dCas9-CDA1-UGI-attR1
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SpeciesSynthetic
- Promoter RecA promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer 5-TGTAAAACGACGGCCAGT-3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEN-dCas9-CDA1-UGI was a gift from Bing Yang (Addgene plasmid # 196248 ; http://n2t.net/addgene:196248 ; RRID:Addgene_196248) -
For your References section:
Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria. Li C, Wang L, Cseke LJ, Vasconcelos F, Huguet-Tapia JC, Gassmann W, Pauwels L, White FF, Dong H, Yang B. Commun Biol. 2023 Jan 17;6(1):56. doi: 10.1038/s42003-023-04451-8. 10.1038/s42003-023-04451-8 PubMed 36646768
