Skip to main content
Addgene

pRM055 His-TEV-Cas9 6xNLS 0C
(Plasmid #196246)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196246 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pML-2CT
  • Backbone manufacturer
    Macrolab, UC Berkeley
  • Backbone size w/o insert (bp) 5950
  • Total vector size (bp) 9111
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    Cas9-6xNLS
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4103
  • Promoter T7

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    4xNLS-pMJ915v2 from Jennifer Doudna (Addgene plasmid # 88917 ; http://n2t.net/addgene:88917 ; RRID:Addgene_88917)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRM055 His-TEV-Cas9 6xNLS 0C was a gift from Ross Wilson (Addgene plasmid # 196246 ; http://n2t.net/addgene:196246 ; RRID:Addgene_196246)

  • For your References section:

    Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes. Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC. Nat Biomed Eng. 2023 May;7(5):647-660. doi: 10.1038/s41551-023-01032-2. Epub 2023 Apr 25. 10.1038/s41551-023-01032-2 PubMed 37147433
Related items: