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Addgene

pgRNA_AT
(Plasmid #196243)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196243 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript/ pgRNA
  • Modifications to backbone
    Cloned sgRNA cloning cassette with ccdB in pBluscript
  • Vector type
    Bacterial Expression, CRISPR
  • Promoter J23119

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Wang Y., Liu Y., Liu J., Guo Y., Fan L., Ni X., Zheng X., Wang M., Zheng P., Sun J., et al. // Metabolic Engineering. 2018. V. 47. P. 200–210.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pgRNA_AT was a gift from Andrea Puhar (Addgene plasmid # 196243 ; http://n2t.net/addgene:196243 ; RRID:Addgene_196243)

  • For your References section:

    CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in Shigella flexneri by Cytosine Base Editing. Sharma A, Omer Aden R, Puhar A, Cisneros DA. mSystems. 2022 Dec 21:e0104522. doi: 10.1128/msystems.01045-22. 10.1128/msystems.01045-22 PubMed 36541764
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