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Addgene

pnCas9-AID
(Plasmid #196242)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSU19
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    nickase Cas9 fused to AID
  • Promoter tac promoter

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    1. Wang Y., Liu Y., Liu J., Guo Y., Fan L., Ni X., Zheng X., Wang M., Zheng P., Sun J., et al. // Metabolic Engineering. 2018. V. 47. P. 200–210.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pnCas9-AID was a gift from Andrea Puhar (Addgene plasmid # 196242 ; http://n2t.net/addgene:196242 ; RRID:Addgene_196242)

  • For your References section:

    CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in Shigella flexneri by Cytosine Base Editing. Sharma A, Omer Aden R, Puhar A, Cisneros DA. mSystems. 2022 Dec 21:e0104522. doi: 10.1128/msystems.01045-22. 10.1128/msystems.01045-22 PubMed 36541764
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