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Addgene

GB1-A11(2-196, G189E)-Strep
(Plasmid #196212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Backbone size w/o insert (bp) 5633
  • Total vector size (bp) 6527
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Annexin A11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    894
  • Mutation
    G189E
  • Entrez Gene
    ANXA11 (a.k.a. ALS23, ANX11, CAP-50, CAP50, IBMWMA)
  • Tags / Fusion Proteins
    • GB1-6xHis-TEV (N terminal on insert)
    • Strep-II tag (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GB1-A11(2-196, G189E)-Strep was a gift from Lalit Deshmukh (Addgene plasmid # 196212 ; http://n2t.net/addgene:196212 ; RRID:Addgene_196212)

  • For your References section:

    ALS Variants of Annexin A11's Proline-Rich Domain Impair Its S100A6-Mediated Fibril Dissolution. Shihora A, Elias RD, Hammond JA, Ghirlando R, Deshmukh L. ACS Chem Neurosci. 2023 Jul 11. doi: 10.1021/acschemneuro.3c00169. 10.1021/acschemneuro.3c00169 PubMed 37433222
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