GB1-A11(2-196, G38R)-Strep
(Plasmid
#196209)
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PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-11a
- Backbone size w/o insert (bp) 5633
- Total vector size (bp) 6527
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAnnexin A11
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SpeciesH. sapiens (human)
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Insert Size (bp)894
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MutationG38R
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Entrez GeneANXA11 (a.k.a. ALS23, ANX11, CAP-50, CAP50, IBMWMA)
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Tags
/ Fusion Proteins
- GB1-6xHis-TEV (N terminal on insert)
- Strep-II tag (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GB1-A11(2-196, G38R)-Strep was a gift from Lalit Deshmukh (Addgene plasmid # 196209 ; http://n2t.net/addgene:196209 ; RRID:Addgene_196209) -
For your References section:
ALS Variants of Annexin A11's Proline-Rich Domain Impair Its S100A6-Mediated Fibril Dissolution. Shihora A, Elias RD, Hammond JA, Ghirlando R, Deshmukh L. ACS Chem Neurosci. 2023 Jul 11. doi: 10.1021/acschemneuro.3c00169. 10.1021/acschemneuro.3c00169 PubMed 37433222