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Addgene

pTPT
(Plasmid #1962)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19M
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TPT
  • Species
    unknown
  • Insert Size (bp)
    150
  • Mutation
    2 copies of 20bp artificial nucleosome positioning sequence upstream of a TATA box

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 reverse?
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ds oligo containing 2 Shrader and Crothers GT phasing sequences at 5' end and a consensus TATA from AdMLP (about 45bp from 3' end) cloned into Mlu/Eco cut pUC19M. See sequence of insert under "Author's Map." Entered by Anthony Imbalzano. (kingston #868)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTPT was a gift from Robert Kingston (Addgene plasmid # 1962 ; http://n2t.net/addgene:1962 ; RRID:Addgene_1962)

  • For your References section:

    Mitotic inactivation of a human SWI/SNF chromatin remodeling complex. Sif S, Stukenberg PT, Kirschner MW, Kingston RE. Genes Dev 1998 Sep 15;12(18):2842-51. 10.1101/gad.12.18.2842 PubMed 9744861
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