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Addgene

pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro
(Plasmid #196196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHDR_G3BP1-V5-APEX2-GFP
  • Backbone manufacturer
    Eugene Yeo; PubMed 29373831
  • Vector type
    Homology repair donor
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette
  • Species
    H. sapiens (human)
  • Entrez Gene
    G3BP1 (a.k.a. G3BP, HDH-VIII)
  • Tag / Fusion Protein
    • V5-APEX2-mScarlet-I (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In Addgene vector 105284 the GFP was exchanged with the red fluorescent protein mScarlet-I using the plasmid pmScarlet-i_C1 (addgene 85044).

Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro was a gift from Jürgen Knoblich (Addgene plasmid # 196196 ; http://n2t.net/addgene:196196 ; RRID:Addgene_196196)

  • For your References section:

    Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, Durnberger G, Mechtler K, Knoblich JA. Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135. 10.7554/eLife.85135 PubMed 36989136
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