pSpCas9(BB)-2A-tomato-TSC2-sgRNA
(Plasmid #196195)

• Purpose: Editing human TSC2 locus
• Depositing Lab: Jürgen Knoblich
• Publication: Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135.

Backbone

• Vector backbone: PX458
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TSC2
• gRNA/shRNA sequence: unknown
• Species: H. sapiens (human)
• Entrez Gene: TSC2 (a.k.a. LAM, PPP1R160, TSC4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pSpCas9(BB)-2A-tomato-TSC2-sgRNA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloning was performed using pSpCas9(BB)-2A-GFP plasmid (Addgene, 48138, RRID:Addgene_48138), modified to express eCas9 instead of WT-Cas9 and dTomato instead of GFP (Esk et al., 2020), using the Bbs1 cloning strategy of Ran et al. (Ran et al., 2013).24 plasmid (Addgene, 48138, RRID:Addgene_48138), modified to express eCas9 instead of WT-25 Cas9 and dTomato instead of GFP (Esk et al., 2020), using the Bbs1 cloning strategy of Ran26 et al. (Ran et al., 2013).

Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.