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AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
(Plasmid #196192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1-SA-Puro
  • Vector type
    ZNF donor
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For this purpose, a previously generated backbone containing 2xCHS4-EF1a-tdTomato-SV40-2xCHS4 was used (Bagley et al., 2017).

Please visit https://www.biorxiv.org/content/10.1101/2022.10.07.511280v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA was a gift from Jürgen Knoblich (Addgene plasmid # 196192 ; http://n2t.net/addgene:196192 ; RRID:Addgene_196192)

  • For your References section:

    Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, Durnberger G, Mechtler K, Knoblich JA. Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135. 10.7554/eLife.85135 PubMed 36989136
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