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pHDR_sox2HA_P2A-GFP-pA_HA_G2913A
(Plasmid #196191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pF2DTA
  • Vector type
    Homology repair donor

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SOX2-HAL-P2A-EGFP-SOX2-HAR
  • Species
    H. sapiens (human)
  • Mutation
    G2913A mutation to replace the guide cutting site in the Homology construct.
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHDR_sox2HA_P2A-GFP-pA_HA_G2913A was a gift from Jürgen Knoblich (Addgene plasmid # 196191 ; http://n2t.net/addgene:196191 ; RRID:Addgene_196191)

  • For your References section:

    Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids. Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, Durnberger G, Mechtler K, Knoblich JA. Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135. 10.7554/eLife.85135 PubMed 36989136
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