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Addgene

pMSCV_IRES_GFP_CD16a
(Plasmid #196189)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196189 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMSCV Gateway IRES GFP
  • Backbone manufacturer
    Mark Moasser Lab
  • Backbone size w/o insert (bp) 7788
  • Total vector size (bp) 8553
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD16a
  • Alt name
    FCγRIIIa
  • Alt name
    FCGR3A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    765
  • Mutation
    changed Phenylalanine 158 to Valine
  • GenBank ID
    NM_000569.8
  • Entrez Gene
    FCGR3A (a.k.a. CD16, CD16-II, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, FcGRIIIA, IGFR3, IMD20)
  • Promoter LTR

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV_IRES_GFP_CD16a was a gift from Ana Ruiz-Saenz (Addgene plasmid # 196189 ; http://n2t.net/addgene:196189 ; RRID:Addgene_196189)
  • For your References section:

    A co-culture model system to quantify antibody-dependent cellular cytotoxicity in human breast cancer cells using an engineered natural killer cell line. Vincken R, Ruiz-Saenz A. STAR Protoc. 2023 Apr 17;4(2):102224. doi: 10.1016/j.xpro.2023.102224. 10.1016/j.xpro.2023.102224 PubMed 37071532