pMSCV_IRES_GFP_CD16a
(Plasmid #196189)

• Purpose: Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.
• Depositing Lab: Ana Ruiz-Saenz
• Publication: Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. doi: 10.1016/j.xpro.2023.102224.

Backbone

• Vector backbone: pMSCV Gateway IRES GFP
• Backbone manufacturer: Mark Moasser Lab
• Backbone size w/o insert (bp): 7788
• Total vector size (bp): 8553
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD16a
• Alt name: FCγRIIIa
• Alt name: FCGR3A
• Species: H. sapiens (human)
• Insert Size (bp): 765
• Mutation: changed Phenylalanine 158 to Valine
• GenBank ID: NM_000569.8
• Entrez Gene: FCGR3A (a.k.a. CD16, CD16-II, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, FcGRIIIA, IGFR3, IMD20)
• Promoter: LTR

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: Puro-R

Resource Information

• Supplemental Documents:
  • AR741_pMSCV_CD16_puro_IRES_GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry