pEPSW1KN0034
(Plasmid
#196178)
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PurposeMoClo Level 1 luciferase reporter: CaMV35S::TMV::LucF::flag::35sT
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196178 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCK1
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaMV35S::TMV::LucF::flag::35sT
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.02.13.528283 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEPSW1KN0034 was a gift from Nicola Patron (Addgene plasmid # 196178 ; http://n2t.net/addgene:196178 ; RRID:Addgene_196178) -
For your References section:
Tuning Plant Promoters Using a Simple Split Luciferase Method to Assess Transcription Factor-DNA Interactions. Cai YM, Witham S, Patron NJ. ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 10.1021/acssynbio.3c00094. Epub 2023 Oct 19. 10.1021/acssynbio.3c00094 PubMed 37856867