pEPYCdKN0010
(Plasmid
#196151)
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PurposeGateway destination vector for protein expression of 9xHis_AtNLP7_HiBit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196151 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepH9GW
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name9xHis_AtNLP7_HiBit
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SpeciesA. thaliana (mustard weed)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.02.13.528283 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEPYCdKN0010 was a gift from Nicola Patron (Addgene plasmid # 196151 ; http://n2t.net/addgene:196151 ; RRID:Addgene_196151) -
For your References section:
Tuning Plant Promoters Using a Simple Split Luciferase Method to Assess Transcription Factor-DNA Interactions. Cai YM, Witham S, Patron NJ. ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 10.1021/acssynbio.3c00094. Epub 2023 Oct 19. 10.1021/acssynbio.3c00094 PubMed 37856867