AAV-CAG-Cre
(Plasmid #196140)

• Purpose: AAV-mediated expression of Cre under the CAG promoter .
• Depositing Lab: Edward Boyden
• Publication: Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014 Jul 6.

Backbone

• Vector backbone: AAV with CAG promter
• Backbone manufacturer: Scott Sternson
• Backbone size w/o insert (bp): 4728
• Total vector size (bp): 5784
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Can use DH5alpha at 37°C or Stbl3 at 30°C. Carbenicillin is preferred over ampicillin. In DH5alpha this plasmid may act more like a high copy plasmid, although in Stbl3 it may act more like a low copy plasmid.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cre
• Species: Synthetic
• Insert Size (bp): 1056
• Mutation: codon diversified
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ctagagcctctgctaacc
• 3′ sequencing primer: gcagcgtatccacatagcg

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.