pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L
(Plasmid
#196136)
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PurposeSB vector for the mammalian expression of GADD34-K3L
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196136 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSBtet-Hyg
- Backbone size w/o insert (bp) 7664
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGADD34 Δ1-240 - P2A - K3L
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SpeciesH. sapiens (human); vaccinia virus
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Insert Size (bp)1625
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Entrez GenePPP1R15A (a.k.a. GADD34)
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Tag
/ Fusion Protein
- P2A
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L was a gift from Jamie Cate (Addgene plasmid # 196136 ; http://n2t.net/addgene:196136 ; RRID:Addgene_196136) -
For your References section:
A highly efficient human cell-free translation system. Aleksashin NA, Chang ST, Cate JHD. bioRxiv. 2023 Feb 10:2023.02.09.527910. doi: 10.1101/2023.02.09.527910. Preprint. 10.1101/2023.02.09.527910 PubMed 36798401