pHEN2-SG4 R105A
(Plasmid #196072)

• Purpose: Express mutant (R105A) SG4 in E.coli
• Depositing Lab: Shankar Balasubramanian
• Publication: Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/jacs.2c10656.

Backbone

• Vector backbone: pHEN2
• Backbone manufacturer: Hybrigenics Services
• Backbone size w/o insert (bp): 4595
• Total vector size (bp): 4967
• Modifications to backbone: 3xFLAG inserted at C-terminal on backbone, 3xMYC removed from C-terminal on backbone
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Must be transformed into BL21(DE3) bacteria and grown at 28°C for antibody production.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SG4 R105A
• Species: Synthetic
• Mutation: R105A mutation in SG4
• Promoter: T7
• Tags / Fusion Proteins:
  • 6XHis (C terminal on backbone)
  • 3XFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GAA TTG TGA GCG GAT AAC
• 3′ sequencing primer: CAG CCC TCA TAG TTA GCG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Hybrigenics Services

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry