YW249_Pzipt17-loxP-H2BGFP-miniMOS
(Plasmid #196063)

• Purpose: a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.
• Depositing Lab: Wenxing Yang
• Publication: Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.7717/peerj.15433. eCollection 2023.

Backbone

• Vector backbone: pCFJ910
• Backbone size w/o insert (bp): 5496
• Total vector size (bp): 12691
• Vector type: Worm Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: H2B::GFP
• Species: C. elegans (nematode)

Gene/Insert 2

• Gene/Insert name: NeoR
• Species: C. elegans (nematode)

Gene/Insert 3

• Gene/Insert name: Minimal Mos1
• Species: D. melanogaster (fly), C. elegans (nematode)

Gene/Insert 4

• Gene/Insert name: zipt17p::zipt17::zipt17 3UTR
• Species: C. elegans (nematode)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry