Rosa26-LSL-PE2 Targeting Vector
(Plasmid
#196038)
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PurposeThe targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196038 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLSL-Cas9-Rosa26TV
- Backbone size w/o insert (bp) 12710
- Total vector size (bp) 22662
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Vector typeMammalian Expression, Mouse Targeting
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Selectable markersNeomycin (select with G418) ; DTA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas9-MMLV-RT
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Alt namePE2
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SpeciesSynthetic; Streptococcus pyogenes (cas9) and MMLV (MMLV RT)
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Insert Size (bp)7125
- Promoter CAGG
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AAGCTAGATCGAATTCGGCCGGCCTTCGAAgccaccatgaaacggacagc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rosa26-LSL-PE2 Targeting Vector was a gift from Tyler Jacks (Addgene plasmid # 196038 ; http://n2t.net/addgene:196038 ; RRID:Addgene_196038) -
For your References section:
A prime editor mouse to model a broad spectrum of somatic mutations in vivo. Ely ZA, Mathey-Andrews N, Naranjo S, Gould SI, Mercer KL, Newby GA, Cabana CM, Rideout WM 3rd, Jaramillo GC, Khirallah JM, Holland K, Randolph PB, Freed-Pastor WA, Davis JR, Kulstad Z, Westcott PMK, Lin L, Anzalone AV, Horton BL, Pattada NB, Shanahan SL, Ye Z, Spranger S, Xu Q, Sanchez-Rivera FJ, Liu DR, Jacks T. Nat Biotechnol. 2023 May 11. doi: 10.1038/s41587-023-01783-y. 10.1038/s41587-023-01783-y PubMed 37169967
