pCM3.06
(Plasmid
#19602)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19602 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR201
- Backbone size w/o insert (bp) 2257
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namefbf-1 ORF+3' in pDONR201
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SpeciesC. elegans (nematode)
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Insert Size (bp)2490
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GenBank IDNM_062815
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Entrez Genefbf-1 (a.k.a. CELE_H12I13.4)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attP1 (destroyed during cloning)
- 3′ cloning site attP2 (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM3.06 was a gift from Geraldine Seydoux (Addgene plasmid # 19602 ; http://n2t.net/addgene:19602 ; RRID:Addgene_19602) -
For your References section:
Conserved regulation of MAP kinase expression by PUF RNA-binding proteins. Lee MH, Hook B, Pan G, Kershner AM, Merritt C, Seydoux G, Thomson JA, Wickens M, Kimble J. PLoS Genet. 2007 Dec 28. 3(12):e233. 10.1371/journal.pgen.0030233 PubMed 18166083