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Addgene

pCM3.06
(Plasmid #19602)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19602 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDONR201
  • Backbone size w/o insert (bp) 2257
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    fbf-1 ORF+3' in pDONR201
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2490
  • GenBank ID
    NM_062815
  • Entrez Gene
    fbf-1 (a.k.a. CELE_H12I13.4)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP1 (destroyed during cloning)
  • 3′ cloning site attP2 (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM3.06 was a gift from Geraldine Seydoux (Addgene plasmid # 19602 ; http://n2t.net/addgene:19602 ; RRID:Addgene_19602)
  • For your References section:

    Conserved regulation of MAP kinase expression by PUF RNA-binding proteins. Lee MH, Hook B, Pan G, Kershner AM, Merritt C, Seydoux G, Thomson JA, Wickens M, Kimble J. PLoS Genet. 2007 Dec 28. 3(12):e233. 10.1371/journal.pgen.0030233 PubMed 18166083