pCM1.112
(Plasmid
#19599)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePDONRP4P1R
- Backbone size w/o insert (bp) 2645
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namefbf-2 prom in PDONRP4P1R
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SpeciesC. elegans (nematode)
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Insert Size (bp)1511
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attP4 (destroyed during cloning)
- 3′ cloning site attP1R (destroyed during cloning)
- 5′ sequencing primer M13Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM1.112 was a gift from Geraldine Seydoux (Addgene plasmid # 19599 ; http://n2t.net/addgene:19599 ; RRID:Addgene_19599)