pCB24 pDEST crybb1:TagBFP
(Plasmid
#195987)
-
Purpose(Empty Backbone) Transgenesis backbone
-
Depositing Labs
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 195987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepDestTol2pA2
-
Vector typeOther
-
Selectable markersccdB
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCB24 pDEST crybb1:TagBFP was a gift from Markus Affolter & Christian Mosimann (Addgene plasmid # 195987 ; http://n2t.net/addgene:195987 ; RRID:Addgene_195987) -
For your References section:
Next-generation plasmids for transgenesis in zebrafish and beyond. Kemmler CL, Moran HR, Murray BF, Scoresby A, Klem JR, Eckert RL, Lepovsky E, Bertho S, Nieuwenhuize S, Burger S, D'Agati G, Betz C, Puller AC, Felker A, Ditrychova K, Botschi S, Affolter M, Rohner N, Lovely CB, Kwan KM, Burger A, Mosimann C. Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531. 10.1242/dev.201531 PubMed 36975217