pAF018 p3E-2A-mCerulean-CAAX
(Plasmid
#195975)
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PurposeGateway p3E 2A fluorophore
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3E
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2A-mCerulean-CAAX (membrane)
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SpeciesOther
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAF018 p3E-2A-mCerulean-CAAX was a gift from Christian Mosimann (Addgene plasmid # 195975 ; http://n2t.net/addgene:195975 ; RRID:Addgene_195975) -
For your References section:
Cre/lox-controlled spatiotemporal perturbation of FGF signaling in zebrafish. Kirchgeorg L, Felker A, van Oostrom M, Chiavacci E, Mosimann C. Dev Dyn. 2018 Oct;247(10):1146-1159. doi: 10.1002/dvdy.24668. 10.1002/dvdy.24668 PubMed 30194800