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Addgene

457 p3E-2A-EGFPpA
(Plasmid #195967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195967 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/D
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2A-EGFP
  • Species
    Other

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    457 p3E-2A-EGFPpA was a gift from Kristen Kwan & Christian Mosimann (Addgene plasmid # 195967 ; http://n2t.net/addgene:195967 ; RRID:Addgene_195967)

  • For your References section:

    Next-generation plasmids for transgenesis in zebrafish and beyond. Kemmler CL, Moran HR, Murray BF, Scoresby A, Klem JR, Eckert RL, Lepovsky E, Bertho S, Nieuwenhuize S, Burger S, D'Agati G, Betz C, Puller AC, Felker A, Ditrychova K, Botschi S, Affolter M, Rohner N, Lovely CB, Kwan KM, Burger A, Mosimann C. Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531. 10.1242/dev.201531 PubMed 36975217
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