pCK068 pME minprom_mApple
(Plasmid
#195963)
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PurposeGateway pME fluorophore. *Note: this plasmid has been discontinued. Please see the new, optimized version, Plasmid 213483.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195963 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR/D
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemouse beta-globin minimal promoter mApple
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SpeciesOther
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCK068 pME minprom_mApple was a gift from Christian Mosimann (Addgene plasmid # 195963) -
For your References section:
Next-generation plasmids for transgenesis in zebrafish and beyond. Kemmler CL, Moran HR, Murray BF, Scoresby A, Klem JR, Eckert RL, Lepovsky E, Bertho S, Nieuwenhuize S, Burger S, D'Agati G, Betz C, Puller AC, Felker A, Ditrychova K, Botschi S, Affolter M, Rohner N, Lovely CB, Kwan KM, Burger A, Mosimann C. Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531. 10.1242/dev.201531 PubMed 36975217