L1P63
(Plasmid
#195938)
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PurposePhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35S
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195938 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGII-L1-Rep
- Backbone size w/o insert (bp) 4708
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepUBQ10-PI-tUBQ1-p35S-IP-t35S
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SpeciesSynthetic
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Insert Size (bp)6460
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IS4 Transposon present in plasmid backbone. This is a known insertion and is not of any functional concern.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L1P63 was a gift from Jennifer Nemhauser (Addgene plasmid # 195938 ; http://n2t.net/addgene:195938 ; RRID:Addgene_195938) -
For your References section:
An integrase toolbox to record gene-expression during plant development. Guiziou S, Maranas CJ, Chu JC, Nemhauser JL. Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5. 10.1038/s41467-023-37607-5 PubMed 37012288