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Addgene

L1P59
(Plasmid #195936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGII-L1-Rep
  • Backbone size w/o insert (bp) 4708
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p35S-PI-t35S
  • Species
    Synthetic
  • Insert Size (bp)
    3802

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IS4 Transposon present in plasmid backbone. This is a known insertion and is not of any functional concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L1P59 was a gift from Jennifer Nemhauser (Addgene plasmid # 195936 ; http://n2t.net/addgene:195936 ; RRID:Addgene_195936)

  • For your References section:

    An integrase toolbox to record gene-expression during plant development. Guiziou S, Maranas CJ, Chu JC, Nemhauser JL. Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5. 10.1038/s41467-023-37607-5 PubMed 37012288
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