pNL2119 pARF7 GG0-GG1
(Plasmid
#195910)
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PurposeLevel 0 pARF7 promoter with GG0 and GG1 golden gate linkers
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195910 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR-BluntII-TOPO
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 3512
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepARF7
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2038
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Entrez GeneNPH4 (a.k.a. AT5G20730, ARF7, AUXIN RESPONSE FACTOR 7, AUXIN-REGULATED TRANSCRIPTIONAL ACTIVATOR 7, AUXIN-RESPONSIVE TRANSCRIPTIONAL ACTIVATOR 7, BIP, BIPOSTO, IAA21, IAA23, IAA25, MASSUGU 1, MSG1, NON-PHOTOTROPHIC HYPOCOTYL, T1M15.130, T1M15_130, TIR5, TRANSPORT INHIBITOR RESPONSE 5, indole-3-acetic acid inducible 21, indole-3-acetic acid inducible 23, indole-3-acetic acid inducible 25)
Cloning Information
- Cloning method TOPO Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNL2119 pARF7 GG0-GG1 was a gift from Jennifer Nemhauser (Addgene plasmid # 195910 ; http://n2t.net/addgene:195910 ; RRID:Addgene_195910) -
For your References section:
An integrase toolbox to record gene-expression during plant development. Guiziou S, Maranas CJ, Chu JC, Nemhauser JL. Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5. 10.1038/s41467-023-37607-5 PubMed 37012288