pSB74
(Plasmid
#19591)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19591 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTrc99A
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Backbone manufacturerE. Amann
- Backbone size w/o insert (bp) 4176
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namethiosulfate reductase
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Alt namephsABC
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SpeciesS. enterica serovar Typhimurium LT2
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Insert Size (bp)5136
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GenBank IDCP000026
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Entrez GenephsB
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer M13/pUC Reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSB74 was a gift from Jay Keasling (Addgene plasmid # 19591 ; http://n2t.net/addgene:19591 ; RRID:Addgene_19591) -
For your References section:
Engineering hydrogen sulfide production and cadmium removal by expression of the thiosulfate reductase gene (phsABC) from Salmonella enterica serovar typhimurium in Escherichia coli. Bang SW, Clark DS, Keasling JD. Appl Environ Microbiol. 2000 Sep . 66(9):3939-44. 10.1128/AEM.66.9.3939-3944.2000 PubMed 10966412