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L0-I40 Intein-PhiC31 GG1-GG4
(Plasmid #195906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-BluntII-TOPO
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 3512
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Intein-PhiC31
  • Species
    Synthetic
  • Insert Size (bp)
    1052

Cloning Information

  • Cloning method TOPO Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L0-I40 Intein-PhiC31 GG1-GG4 was a gift from Jennifer Nemhauser (Addgene plasmid # 195906 ; http://n2t.net/addgene:195906 ; RRID:Addgene_195906)

  • For your References section:

    An integrase toolbox to record gene-expression during plant development. Guiziou S, Maranas CJ, Chu JC, Nemhauser JL. Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5. 10.1038/s41467-023-37607-5 PubMed 37012288
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