pBABE BRG1
(Plasmid #1959)

• Depositing Lab: Robert Kingston
• Publication: Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BRG1
• Alt name: BAF190a
• Species: H. sapiens (human)
• Insert Size (bp): 5000
• Mutation: E1262K R1575Q
• Entrez Gene: SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
• Tag / Fusion Protein:
  • flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector: pBabe linearized with SalI and CIP'd. Insert: pBS-CeBRG1 digested with SalI to liberate entire flag tagged BRG1 seq. (Kingston #1359)