L0-I14 DST GG4-GG5
(Plasmid
#195896)
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PurposeLevel 0 DST (destabilization RNA tag: SAUR tag) with GG4 GG5 golden gate linkers
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR-BluntII-TOPO
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 3512
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDST
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SpeciesSynthetic
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Insert Size (bp)171
Cloning Information
- Cloning method TOPO Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L0-I14 DST GG4-GG5 was a gift from Jennifer Nemhauser (Addgene plasmid # 195896 ; http://n2t.net/addgene:195896 ; RRID:Addgene_195896) -
For your References section:
An integrase toolbox to record gene-expression during plant development. Guiziou S, Maranas CJ, Chu JC, Nemhauser JL. Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5. 10.1038/s41467-023-37607-5 PubMed 37012288