L0-T6 Empty target Bxb1
(Plasmid
#195889)
-
PurposeLevel 0 Empty Bxb1 target: attP_Bxb1-BpiI-attB_Bxb1 with golden gate linker in pBLUNT plasmid.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 195889 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCR-BluntII-TOPO
-
Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 3512
-
Vector typeSynthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEmpty Bxb1 Target
-
SpeciesSynthetic
-
Insert Size (bp)205
Cloning Information
- Cloning method TOPO Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
L0-T6 Empty target Bxb1 was a gift from Jennifer Nemhauser (Addgene plasmid # 195889 ; http://n2t.net/addgene:195889 ; RRID:Addgene_195889) -
For your References section:
An integrase toolbox to record gene-expression during plant development. Guiziou S, Maranas CJ, Chu JC, Nemhauser JL. Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5. 10.1038/s41467-023-37607-5 PubMed 37012288