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Addgene

TRE-VEGFA EF1a-rtTA3
(Plasmid #195881)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195881 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSpCTRE-CD4
  • Modifications to backbone
    Replace Cas9 with mVEGFA and removed CD4
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VEGFA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Vegfa (a.k.a. L-VEGF, Vegf, Vpf)
  • Promoter TRE3GS

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRE-VEGFA EF1a-rtTA3 was a gift from Elaine Fuchs (Addgene plasmid # 195881 ; http://n2t.net/addgene:195881 ; RRID:Addgene_195881)

  • For your References section:

    Ras drives malignancy through stem cell crosstalk with the microenvironment. Yuan S, Stewart KS, Yang Y, Abdusselamoglu MD, Parigi SM, Feinberg TY, Tumaneng K, Yang H, Levorse JM, Polak L, Ng D, Fuchs E. Nature. 2022 Nov 30. doi: 10.1038/s41586-022-05475-6. 10.1038/s41586-022-05475-6 PubMed 36450983
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