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Addgene

BDH2_FL_BioN
(Plasmid #195870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFBD-BirA
  • Backbone manufacturer
    pFastBac Dual Modified
  • Vector type
    pFBD-BirA, Baculovirus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BDH2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    735
  • Entrez Gene
    BDH2 (a.k.a. DHRS6, EFA6R, PRO20933, SDR15C1, UCPA-OR, UNQ6308)
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • MSGLNDIFEAQKIEWHEGSAGGSG (N terminal on insert)
    • GGSGHHHHHH (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SGC structures for NIH Treat-AD Project

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BDH2_FL_BioN was a gift from Cheryl Arrowsmith (Addgene plasmid # 195870 ; http://n2t.net/addgene:195870 ; RRID:Addgene_195870)
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