pAG8Ha-His-EWSR1-359-549
(Plasmid
#195868)
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PurposeExpresses the RRM-RG2-ZnF (359-549) of EWS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAG8Ha-His
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Backbone manufacturermodified pET vector
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEWS 359-549
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Alt nameEWSR1
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SpeciesH. sapiens (human)
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Insert Size (bp)570
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Entrez GeneEWSR1 (a.k.a. EWS, EWS-FLI1, bK984G1.4)
- Promoter T7
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Tag
/ Fusion Protein
- His8-linker-TEV (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KasI (destroyed during cloning)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert is codon optimized for E. coli expression
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG8Ha-His-EWSR1-359-549 was a gift from David Libich (Addgene plasmid # 195868 ; http://n2t.net/addgene:195868 ; RRID:Addgene_195868) -
For your References section:
Biochemical and biophysical characterization of the nucleic acid binding properties of the RNA/DNA binding protein EWS. Selig EE, Bhura R, White MR, Akula S, Hoffman RD, Tovar CN, Xu X, Booth RE, Libich DS. Biopolymers. 2023 May;114(5):e23536. doi: 10.1002/bip.23536. Epub 2023 Mar 17. 10.1002/bip.23536 PubMed 36929870