pHAGE2-EF1aL-dsRed-W
(Plasmid #195863)

• Purpose: (Empty Backbone) lentiviral expression of the red fluorescent protein driven by human EF1aL promoter
• Depositing Lab: Darrell Kotton
• Publication: lentiviral expression of dsRed driven by human EF1aL promoter (unpublished)

Backbone

• Vector backbone: pHAGE2
• Backbone manufacturer: Darrell Kotton
• Backbone size (bp): 7655
• Vector type: Lentiviral
• Promoter: EF1aL
• Tag / Fusion Protein:
  • dsRed (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pHAGE2-EF1aL-DsRed-W.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pHAGE2 vector was modified as follows: cDNA encoding a variant of the red fluorescent protein adapted from Discosoma sp. (DsRed-Express; Clontech, Mountain View, CA) was amplified by PCR attaching NotI and BamH1 restriction sites to 5' and 3' ends, respectively. This amplicon was cloned into the pHAGE2 backbone in the gene expression position by ligation to NotI/BamH1 cohesive ends. Immediately upstream of the dsRed ATG start site, the Human Translation elongation factor 1A long [Ef1aL], 1185 bp was inserted by standard cloning techniques.