pRCPam
(Plasmid #195861)

• Purpose: Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.
• Depositing Lab: Gregory Phillips
• Publication: DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3):e00094-23. doi: 10.1128/jmbe.00094-23. eCollection 2023 Dec.

Backbone

• Vector backbone: pDHC
• Backbone manufacturer: Reference for backbone - PMID: 10723548
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Amber Chromogenic Protein on pRCPam plasmid
• Alt name: CP62am
• Species: Acropora millepora
• Mutation: Gln62X

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry