pBAD-6xHis-AviTag-ALFAtag-eGFP
(Plasmid
#195854)
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PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195854 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name6xHis-AviTag-ALFAtag-eGFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-6xHis-AviTag-ALFAtag-eGFP was a gift from John Ngo (Addgene plasmid # 195854 ; http://n2t.net/addgene:195854 ; RRID:Addgene_195854) -
For your References section:
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection. Sloas DC, Tran JC, Marzilli AM, Ngo JT. Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01638-y. 10.1038/s41587-022-01638-y PubMed 36646932