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Addgene

HiBiT-SMO
(Plasmid #195851)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195851 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SMO
  • Species
    M. musculus (mouse)
  • Mutation
    D54N and R690G
  • Entrez Gene
    Smo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
  • Promoter CMV
  • Tag / Fusion Protein
    • HiBiT (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HiBiT-SMO was a gift from Gunnar Schulte (Addgene plasmid # 195851 ; http://n2t.net/addgene:195851 ; RRID:Addgene_195851)

  • For your References section:

    Residue 6.43 defines receptor function in class F GPCRs. Turku A, Schihada H, Kozielewicz P, Bowin CF, Schulte G. Nat Commun. 2021 Jun 24;12(1):3919. doi: 10.1038/s41467-021-24004-z. 10.1038/s41467-021-24004-z PubMed 34168128
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