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Addgene

PAGFP_NS2B3
(Plasmid #195755)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195755 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PAGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dengue (Type-2) NS2B3
  • Species
    Dengue Virus Serotype 2, Strain NGC
  • Insert Size (bp)
    2355
  • Mutation
    No mutations
  • Promoter CMV
  • Tag / Fusion Protein
    • PA-GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PAGFP_NS2B3 was a gift from Partha Mondal (Addgene plasmid # 195755 ; http://n2t.net/addgene:195755 ; RRID:Addgene_195755)
  • For your References section:

    Single-Molecule Super-Resolution Microscopy Reveals Formation of NS2B3 Protein Clusters on Mitochondrial Network Leading to its Fragmentation during the Onset of Dengue (Denv-2) Viral Infection. Varghese JM, Joshi P, Aravinth S, Mondal PP. bioRxiv 2022.12.18.520514 10.1101/2022.12.18.520514