pGas-miRFP718nano
(Plasmid
#195752)
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PurposeG protein alpha-subunit internally tagged with miRFP718nano
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195752 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameG protein alpha-s-miRFP718nano
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1626
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGas-miRFP718nano was a gift from Vladislav Verkhusha (Addgene plasmid # 195752 ; http://n2t.net/addgene:195752 ; RRID:Addgene_195752) -
For your References section:
Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein. Oliinyk OS, Ma C, Pletnev S, Baloban M, Toboada C, Sheng H, Yao J, Verkhusha VV. Nat Methods. 2022 Dec 1. doi: 10.1038/s41592-022-01683-0. 10.1038/s41592-022-01683-0 PubMed 36456785