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Addgene

pBAD/His-miRFP718nano
(Plasmid #195743)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195743 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/His-B
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miRFP718nano
  • Species
    Synthetic
  • Insert Size (bp)
    441
  • Promoter araC
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBAD-fwd
  • 3′ sequencing primer pBAD-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modifications to backbone: Several amino acids between His-tag and miRFP718nano were removed

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD/His-miRFP718nano was a gift from Vladislav Verkhusha (Addgene plasmid # 195743 ; http://n2t.net/addgene:195743 ; RRID:Addgene_195743)

  • For your References section:

    Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein. Oliinyk OS, Ma C, Pletnev S, Baloban M, Toboada C, Sheng H, Yao J, Verkhusha VV. Nat Methods. 2022 Dec 1. doi: 10.1038/s41592-022-01683-0. 10.1038/s41592-022-01683-0 PubMed 36456785
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