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Addgene

pET (SUMO)-Syt9
(Plasmid #195713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 for protein expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Syt9
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Syt5 (a.k.a. SytIX)
  • Tag / Fusion Protein
    • GST-SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

amino terminal GST followed by thrombin cleavage site followeed by SUMO folowed by full length SYT9

Please visit https://www.biorxiv.org/content/10.1101/2022.04.18.488681v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET (SUMO)-Syt9 was a gift from Edwin Chapman (Addgene plasmid # 195713 ; http://n2t.net/addgene:195713 ; RRID:Addgene_195713)

  • For your References section:

    Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion. Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER. J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2. 10.1523/JNEUROSCI.1857-22.2023 PubMed 36732068
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