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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1957 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFastBac1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4775
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Vector typeInsect Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBRG1
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Alt nameBAF190a
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SpeciesH. sapiens (human)
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Entrez GeneSMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
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Tag
/ Fusion Protein
- flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A C-terminal Flag tag was added to BRG1 by PCR using a 3? primer designed to incorporate the FLAG sequence (DYKDDDDK) before the stop codon, followed by SalI and SpeI restriction sites. The PCR product was digested with BglII and SpeI and cloned into pBS/Brg1. The full-length Brg1 cDNA was then excised out of pBS/FL-Brg1 as a SalI fragment and cloned into pFastBac1 to generate pFastBac1/Brg1(Flag). (kingston #1306)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac1 Flag BRG1 was a gift from Robert Kingston (Addgene plasmid # 1957 ; http://n2t.net/addgene:1957 ; RRID:Addgene_1957) -
For your References section:
Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits. Phelan ML, Sif S, Narlikar GJ, Kingston RE. Mol Cell 1999 Feb;3(2):247-53. 10.1016/S1097-2765(00)80315-9 PubMed 10078207
Map uploaded by the depositor.