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Addgene

pBEL2543
(Plasmid #195664)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 195664 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MlaC
  • Species
    E. coli
  • Mutation
    MlaC(V146R)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEL2543 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 195664 ; http://n2t.net/addgene:195664 ; RRID:Addgene_195664)

  • For your References section:

    Protein-protein interactions in the Mla lipid transport system probed by computational structure prediction and deep mutational scanning. MacRae MR, Puvanendran D, Haase MAB, Coudray N, Kolich L, Lam C, Baek M, Bhabha G, Ekiert DC. J Biol Chem. 2023 Apr 24:104744. doi: 10.1016/j.jbc.2023.104744. 10.1016/j.jbc.2023.104744 PubMed 37100290
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