pBEL2403
(Plasmid
#195642)
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PurposeComplementation of MlaC(WT)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 195642 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMlaC
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SpeciesE. coli
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.12.09.519820v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEL2403 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 195642 ; http://n2t.net/addgene:195642 ; RRID:Addgene_195642) -
For your References section:
Protein-protein interactions in the Mla lipid transport system probed by computational structure prediction and deep mutational scanning. MacRae MR, Puvanendran D, Haase MAB, Coudray N, Kolich L, Lam C, Baek M, Bhabha G, Ekiert DC. J Biol Chem. 2023 Apr 24:104744. doi: 10.1016/j.jbc.2023.104744. 10.1016/j.jbc.2023.104744 PubMed 37100290